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Image Search Results
Journal: Journal of Pain Research
Article Title: Long Non-Coding RNA and mRNA Profiles in the Spinal Cord of Rats with Resiniferatoxin-Induced Neuropathic Pain
doi: 10.2147/JPR.S368599
Figure Lengend Snippet: The expression profile analysis of the lncRNAs/mRNAs and overview of microarray analysis. The purity and quantity of RNA were evaluated by a K5500 micro-spectrophotometer. RNA purity was acceptable if A260/A230 ≥ 0.5 and A260/A280 ≥ 1.5. RNA integrity was acceptable when RIN value ≥ 7 detected by Agilent 2200 RNA assay. Gel electrophoresis ( A ) was conducted to assess the genomic DNA contamination. Expression signal distribution after every mRNA array dataset was normalized ( B ). The microarray heat map of spinal dorsal horn tissues in the PHN model and analysis of differentially expressed lncRNAs (Vehicle group: A1, A2 and A3; PHN model group: B1, B2 and B3) ( C and D ). Red color represented that the relative expression was high and green color represented that the relative expression was low. For advanced data analysis, all biological replicates were pooled and calculated to identify differentially expressed mRNAs based on the threshold of |Fold change| ≥ 1.5 and P ≤ 0.05. Unsupervised hierarchical clustering analysis was conducted to demonstrate the correlation of expression profiles between treatment and biological replicates.
Article Snippet: The
Techniques: Expressing, Microarray, Spectrophotometry, Nucleic Acid Electrophoresis
Journal: Journal of Pain Research
Article Title: Long Non-Coding RNA and mRNA Profiles in the Spinal Cord of Rats with Resiniferatoxin-Induced Neuropathic Pain
doi: 10.2147/JPR.S368599
Figure Lengend Snippet: LncRNA-mRNA interaction network diagram. The red box represents lncRNAs, the blue circle represents predicted mRNAs, and the line represents the relationship between lncRNAs and mRNAs. All LncRNA target genes were obtained by LncRNA target gene prediction. The interaction between lncRNAs and target genes was obtained. LncRNA-mRNA interaction network diagram was drawn. Due to the large number of results of this project, lncRNAs with more than 20 target genes were screened ( A ). A network diagram of the relationships between overlapping genes. The intersection of mRNAs predicted by lncRNAs and differential mRNA results was revealed, and the interaction network diagram between the intersection genes and the corresponding lncRNA was drawn ( B ). An overview of the aberrant lncRNAs by analyzing the microarray data ( C ).
Article Snippet: The
Techniques: Microarray
Journal: Redox Report : Communications in Free Radical Research
Article Title: LncRNA Fendrr: involvement in the protective role of nucleolin against H 2 O 2 -induced injury in cardiomyocytes
doi: 10.1080/13510002.2023.2168626
Figure Lengend Snippet: lncRNAs regulated by nucleolin in cardiomyocytes. (A) Heatmap profile of lncRNA microarray analysis. Green to red colors indicate low to high transcriptional levels. The lncRNAs differentially expressed between the two groups were identified through paired t-test P ≤ 0.05 and a fold change (FC) ≥ 2.5; n = 3 independent biological samples for each group. (B) The expression of 15 selected lncRNAs in chip test, where 10 differentially expressed up-regulated lncRNAs (up) and 5 differentially expressed down-regulated lncRNAs (down) were selected. (C) The interactions between nucleolin and lncRNAs were confirmed by RIP and identified via qRT-PCR. *, P < 0.05, vs. IgG group, n = 3. pcDNA3.1, the empty vector served as negative control; pcDNA3.1-Nuc, overexpression nucleolin group. (D) Bioinformatics website predicted Fendrr binding elements with nucleolin.
Article Snippet: Using the Aglient
Techniques: Microarray, Expressing, Quantitative RT-PCR, Plasmid Preparation, Negative Control, Over Expression, Binding Assay